Mass Spectrometry (MSI)
University of Salerno
Bruker TimsTofPro
Trapped ion mobility mass spectrometry has emerged as powerful technique for metabolomics and lipidomics, as an additional dimension it separates molecules based on their mobility, hence isomers coeluted on chromatograpy dimension can be separated and collision cross section values increase confidence in metabolite/lipidi annotation. Parallel accumulation serial fragmentation (PASEF) allows to increase the numbers of precursors while keeping pace with very fast chromatography (up to 100 Hz in MS/MS). This instrumenti is a quadrupole-TOF that allows to dig deeper in biological matrices such as plasma and tissue for metabolite/lipids characterization.
Configuration
Coupled to UHPLC Ultimate 3000 RSLC
ESI interface, optional APCI
External and internal calibration possible
Properties
Max resolution: 35000
MS/MS modes: DDA, DIA, PASEF, PRM, bbCID
CID fragmentation
Mass accuracy <1 ppm
Analyzer: Quadrupole Time of Flight
Softwares
MetaboScape 2021b for alignment, annotation and filtering
Data Analysis for raw data analysis
Workstation: HP Xeon 3.60GHz, 64 GB RAM
Thermo Orbitrap Lumos
High resolution mass spectrometry is now the workhorse of proteomics, to identify and quantify proteins in complex and precious samples, such as cell lysates, organoids, biofluids and biopsies high sensitivity and fast scanning speed are necessaries. Orbitrap mass spectrometers series, with their ultra high resolution and excellent mass accuracy are highly suited for both bottom-up and top-down proteomics, enabling to identify and quantify thousands of proteins in a single run, helping in evaluate protein turnover and modulation in disease models, as well as to study the effect of novel drugs. The present instrument is a hybrid quadrupole-Orbitrap-Ion trap MS platform dedicated to proteomic analysis by both labeling or label free approaches.
Configuration
Coupled to nano UHPLC Ultimate 3000
ESI and nESI interface
External and internal calibration possible
Properties
Max Resolution: 1.000.000
MS/MS modes: DDA, DIA, PRM, SIM
CID, HCD, ETD fragmentation
Analyzer: Ion Trap and Orbitrap
Mass accuracy <1 ppm
Softwares
Proteome Discoverer for alignment, annotation and filtering
Freestyle for raw data analysis
Workstation: HP Xeon 3.60GHz, 64 GB RAM
Bruker RapifleX Tissuetyper
Maldi-mass spectrometry imaging and spatial-omics allow to map the spatial distribution of drugs or biomolecules in tissues, usually mass spectrometry methods rely on the homogenization of tissue and subsequent analysis. This leads to loss of spatial organization of the tissue, thus not providing detailed information of the tissue microenvironment. MALDI mass spectrometry imaging is a label free technique that allows to detect directly on tissue metabolites, lipids, proteins and drugs. Tissues need to be snap-frozen (nitrogen or isopentane) and stored in pre-chilled collection tubes. The instrument is a MALDI-TOF-TOF MS specifically designed for fast and high spatial resolution MALDI-Imaging applications.
Configuration
MALDI source
External calibration
Laser smartbeam™ 3D, laser repetition rate 5kHZ,
< 10 µm spatial resolution
Properties
Max Resolution: 40.000
MS/MS modes: LIFT
CID
Analyzer: TOF-TOF
Mass accuracy <5 ppm
Softwares
SciLs for MSI imaging
FlexImaging for raw data analysis
Workstation: HP Xeon 3.60GHz, 64 GB RAM
Agilent GCMS 5977b
GC-MS is a high robust method that allows the analysis of complex samples after their derivatization. The employment of electron impact sources allows easy comparison of mass spectra with libraries for identification of metabolites in the samples. This Gas chromatograph-mass spectrometer is the latest in the series of most trusted single quadrupole GC/MS instruments. It is ideal for on applications such as environmental, chemical, petrochemical, food, forensic, pharmaceutical, and material testing.
Configuration
EI source
External calibration
Properties
MS modes: Scan and SIM
Analyzer: Quadrupole
Softwares
MassHunter for raw data analysis
Shimadzu LC40prep-LCMS2020
The isolation of bioactive compounds from complex matrices, such as biofluids, synthesis reaction and phytocomplexes is essential to perform in vivo or in vitro assays. Semi or preparative liquid chromatography assisted by mass spectrometry and UV detection allow to isolate and purify large amount of compounds with relative purities up to 95%. This instrument is a preparative liquid chromatography system equipped with photodiode array and mass spectrometry (single quadrupole).
Configuration
ESI source, Additional detectors: PDA
External calibration
LC: up to 440 bar (prep) 1300 bar (analytical), Flow rate: up to 100 mL/min in prep mode
Properties
MS modes: Scan and SIM
Analyzer: Quadrupole
Softwares
LC-MS solution for raw data analysis
Contacts:
Prof. Pietro Campiglia (tel:)
Prof. Eduardo Maria Sommella (tel:)